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Project Biohacking 101 Edition
N=1/One Life.Optimize It/
Jeffrey Nunn
August 04, 2025
Why "101"?
This is your entry point into a new paradigm where aging becomes a problem to solve, not accept.
Welcome to the first edition of the Project Biohacking Newsletter.
You've just stepped into a world where you become the scientist of your health optimization journey.
This isn't recycled wellness advice. It's your backstage pass to the revolutionary protocols I'm testing, the peptides rewriting aging, and data-driven strategies that separate real biohackers from weekend enthusiasts.
Weekly, I share raw results from my experiments—wins, failures, and discoveries you won't find elsewhere. From peptides to supplement stacks that move biomarkers, get real-world intel that turns theory into results.
This community is built for serious experimenters who test protocols, share data, and move their biomarkers. No fluff, just results.
The experiment begins now.
Table of Contents
📣 Announcements
If you have not listened to Crrow777 Radio’s Episode #637, check it out below. And please share on Social Media!👇🏻
Peptide Coaching is here!
Introducing Project Biohacking Coaching—personalized protocols for serious optimization. Ready to move beyond theory and start seeing real biomarker changes? Use code Crrow50 for 50% off the Biohacking Foundations package. 🤩
Website Updates
I am pushing out content multiple times a week and will let you know in this Newsletter. I have added a Peptide Calculator and plan on adding standard dosages of everyone’s favorites.
Thursday, August 7th @ 5:00 PM CST
Connect with like-minded truth-seekers exploring natural healing⚕️
Join us on Thursday, August 8, 2025, @ 6:00 PM CST
Featured Protocol: NAD+
NAD⁺ Optimization Protocols: A Complete Guide to Boosting Cellular Vitality
NAD⁺ (nicotinamide adenine dinucleotide) powers your mitochondria, sirtuins, and cellular repair. But NAD⁺ declines with age, stress, and inflammation, reducing energy, sleep quality, and resilience.
This guide walks you through science-backed protocols for restoring NAD⁺ through various methods: oral, IV, nasal, and advanced stacks.
🧪 1. Oral NAD⁺ Precursors
Supplement | Dose | Timing | Notes |
|---|---|---|---|
NMN (Nicotinamide Mononucleotide) | 250–750 mg | AM (fasted) | Boosts NAD⁺ directly; crosses the BBB |
NR (Nicotinamide Riboside) | 250–500 mg 1–2x/day | AM or PM | Works best with methyl donors |
TMG (Trimethylglycine) | 500–1,000 mg | With NMN/NR | Prevents methylation burnout |
Resveratrol or Pterostilbene | 100–200 mg | With NMN/NR | Activates sirtuins |
⏱ Cycling Tip: Use 5 days on / 2 days off or 2 months on / 1 month off for best results.
💉 2. Intravenous NAD⁺ (IV Therapy)
250–750 mg IV, 1–2x per week
Duration: 1–2 hours per session
Often stacked with B-complex and glutathione
🧠 Great for detox, cognitive reboot, and mitochondrial fatigue.
💪 3. IM NAD⁺ Injections (Do-it-yourself or Clinic)
50–100 mg, 2–3x per week
Inject into the deltoid or the glute with an insulin syringe
May sting—buffer with lidocaine or dilute in saline
🔄 Faster NAD⁺ restoration than oral forms.
🌬 4. Nasal NAD⁺ or NMN Spray
1–2 sprays per nostril (20–50 mg), 1–2x/day
Fast absorption into the brain
Optional stack: Oxytocin or Semax for neuroregeneration
🔬 5. Advanced Biohacker Stacks
⚙️ CD38 Inhibitor + NAD⁺
Compound | Dose | Notes |
|---|---|---|
78c | 5–10 mg | Most potent CD38 inhibitor (research only) |
Apigenin | 50–100 mg | Plant-based, mild CD38 inhibitor |
Quercetin | 250–500 mg | Synergizes with NMN for NAD⁺ elevation |
🔒 Blocks NAD⁺ degradation at the source (CD38 enzyme).
🔋 Mitochondrial Support
CoQ10 / Ubiquinol – 100–200 mg
PQQ – 10–20 mg
L-Carnitine – 500–1,000 mg
ALA (Alpha Lipoic Acid) – 300–600 mg
🧬 Sirtuin Activation Stack
NAD⁺ Precursor (NMN or NR)
Resveratrol or Pterostilbene
TMG or Methylated B-Complex
Fisetin (Senolytic) – 500 mg, 1–2x/month
📆 Protocol Tracking & Cycling
Strategy | Description |
|---|---|
Pulse Dosing | 5 days on / 2 days off |
Monthly Cycle | 2 months on / 1 month off |
Stack Rotation | Alternate CD38 inhibitors or cofactors monthly |
⚠️ Safety & Notes
Always consult a physician before starting injectable or IV protocols.
Monitor methylation status with long-term NR/NMN use.
Not recommended during pregnancy or active cancer diagnosis.
✅ Summary
Optimizing NAD⁺ can enhance:
🔋 Energy metabolism
🧠 Mental clarity
😴 Sleep and recovery
🔧 Mitochondrial resilience
🧬 DNA repair and healthy aging
Track your response, rotate stacks, and customize protocols based on lifestyle, goals, and lab data.
Learn more about NAD+ on Project Biohacking’s blog post — The Ultimate Guide to NAD+: Your Body's Master Energy Molecule 👇🏻
🔮 Next Issue Preview
Pets & Peptides
Wolverine for DogsI will share with you how I healed my dog, Zeus, with the Wolverine blend. It’s a great story. I will go into the situation and how with petides I saved thousands of dollars. If you have a pet, you won’t want to miss this issue!🐶 |  |
👇🏻👇🏻👇🏻👇🏻👇🏻👇🏻😆😆😆😆😆😆😆😆😆😆😆😆👇🏻👇🏻👇🏻👇🏻👇🏻👇🏻👇🏻👇🏻👇🏻👇🏻👇🏻👇🏻
@tripoverdripski Happy birthday #happybirthday #happy #birthday #spell #magic
🔚 Outro & Final Thoughts
That’s it for this week’s dose of data-driven health optimization.
We’re building something bigger than a newsletter — a community of self-experimenters, thinkers, and trailblazers dedicated to owning their biology. If today’s issue gave you clarity, sparked a question, or challenged your thinking, we’d love to hear from you.
💬 Reply to the poll and let me know what you thought
📲 Share this with a fellow biohacker
🧬 Explore 1:1 coaching or personalized protocols here → Coaching
Until next time,
– Jeff
Founder, Project Biohacking
https://www.projectbiohacking.com/
How would you rate the content in this week’s issue?We’re constantly optimizing Project Biohacking — just like you optimize your health. Tell us how you’re liking the newsletter so far 👇 Your feedback helps shape future topics, improve structure, and make this more valuable for you. |
Note: This content is for educational purposes only and is not medical advice. Always consult a licensed professional before starting any health regimen.